PREreviews of Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic Wounds

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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic Wounds

by Heema K. N. Vyas, Binbin Xia, David Alam, Nicholas P. Gracie, Joanna G. Rothwell, Scott A. Rice, Dee Carter, Patrick J. Cullen, and Anne Mai-Prochnow

Posted: July 27, 2023
Server: bioRxiv
DOI: 10.1101/2023.07.26.550769

Abstract

Healing and treatment of chronic wounds are often complicated due to biofilm formation by pathogens. Here, the efficacy of Plasma Activated Water (PAW) as a pre-treatment strategy has been investigated prior to the application of topical antiseptics polyhexamethylene biguanide, povidone iodine, and MediHoney, which are routinely used to treat chronic wounds. The efficacy of this treatment strategy was determined against biofilms of Escherichia coli formed on a plastic substratum and on a human keratinocyte monolayer substratum used as an in vitro biofilm-skin epithelial cell model. PAW pre-treatment greatly increased the killing efficacy of all the three antiseptics to eradicate the E. coli biofilms formed on the plastic and keratinocyte substrates. However, the efficacy of the combined PAW-antiseptic treatment and single treatments using PAW or antiseptic alone was lower for biofilms formed in the in vitro biofilm-skin epithelial cell model compared to the plastic substratum. Scavenging assays demonstrated that reactive species present within the PAW were largely responsible for its anti-biofilm activity. PAW treatment resulted in significant intracellular RONS accumulation within the E. coli biofilms, while also rapidly acting on the microbial membrane leading to outer membrane permeabilisation and depolarisation. Together, these factors contribute to significant cell death, potentiating the antibacterial effect of the assessed antiseptics.

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PREreview by Liu Xuyu et al. of SG Biofilms and Microbiome Club

Authored by Liu Xuyu, Clarence Sim, Hashmath Fatimah, Samantha Quah, Sreelakshmi Cheruvalli, Hana Marican, and Viduthalai Rasheedkhan Regina of SG Biofilms and Microbiome Club

Reviewed by: Liu Xuyu, Clarence Sim, Hashmath Fatimah, Samantha Quah, Sreelakshmi Cheruvalli, Hana Marican and Viduthalai Rasheedkhan Regina

Singapore Centre for Environmental Life Science Engineering, Nanyang Technological University, Singapore
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