This report presents a case of an adult captive corn snake (Pantherophis guttatus) displaying symptoms of gastrointestinal problems, including anorexia, diarrhea, and lethargy. Diagnostic testing using the Vitec 2 Compact System confirmed the presence of Salmonella Paratyphi B, which poses a health risk to both the snake and the potential for zoonotic transmission to humans. Antimicrobial susceptibility testing revealed resistance to cefalexin, gentamicin, and amikacin, common veterinary antibiotics, but susceptibility to levofloxacin, ceftazidime, ciprofloxacin, and trimethoprim/sulfamethoxazole, which could be used for treatment. This case highlights the zoonotic risks associated with Salmonella in reptiles and the need for regular microbiological monitoring to ensure both reptile health and public safety. It emphasizes the importance of responsible husbandry and veterinary care in preventing the spread of antimicrobial resistance.